The US envoy to the EU, Gordon Sondland, said he “was advised today that the president intends to recall me effective immediately”.
Just hours earlier, Lt Col Alexander Vindman, a top expert on Ukraine, was escorted from the White House.
Mr Trump is said to desire a staff shake-up after senators cleared him in the impeachment case on Wednesday.
In its historic vote, the Senate decided not to remove America’s 45th president from office on charges arising from his dealings with Ukraine.
Lt Col Vindman’s twin brother, Yevgeny Vindman, a senior lawyer for the National Security Council, was also sent back to the Department of the Army on Friday.
What about Mr Sondland’s recall?
In a statement issued by his lawyer, Mr Sondland said: “I was advised today that the president intends to recall me effective immediately as United States ambassador to the European Union.
“I am grateful to President Trump for having given me the opportunity to serve, to Secretary [of State Mike] Pompeo for his consistent support, and to the exceptional and dedicated professionals at the US mission to the European Union.
And how was Vindman removed?
Lt Col Vindman’s counsel, David Pressman, told the BBC his client had been “escorted out of the White House where he has dutifully served his country and his president”.
“There is no question in the mind of any American why this man’s job is over, why this country now has one less soldier serving it at the White House,” said the statement.
“LTC Vindman was asked to leave for telling the truth. His honour, his commitment to right, frightened the powerful.”
It added: “The truth has cost LTC Alexander Vindman his job, his career, and his privacy.”
What did Trump and his administration say?
As he left the executive mansion on Friday for North Carolina, Mr Trump told reporters: “I’m not happy with him [Lt Col Vindman].
“You think I’m supposed to be happy with him? I’m not.”
President Trump has so far not commented further.
According to White House sources, Lt Col Vindman had been expecting a transfer. He was telling colleagues for weeks that he was ready to move back to the defence department, where he still holds active-duty soldier status.
Earlier on Friday, US Defence Secretary Mark Esper told reporters his department welcomes back all of its personnel from assignment.
“And as I said we protect all of our service members from retribution or anything like that,” Mr Esper added.
How did Sondland and Vindman annoy Trump?
Testifying in Congress last November, Mr Sondland was very clear in his testimony that a White House visit by Ukrainian President Volodymyr Zelensky was conditional on Kyiv launching investigations that could be politically helpful to President Trump.
“Was there a quid pro quo [a favour granted in return for something]?” Sondland asked. “As I testified previously, with regard to the requested White House call and White House meeting, the answer is yes.”
Mr Sondland was at that time working with Mr Trump’s personal lawyer, Rudy Giuliani, on Ukrainian policy at the explicit direction of the president.
Lt Col Vindman also testified last November. He said he was “concerned” after hearing Mr Trump’s “improper” phone call on 25 July last year with Ukraine’s president.
The call led to Mr Trump’s impeachment in December by the House for abuse of power and obstruction of Congress.
Democratic lawmakers argued that the president had dangled US aid in exchange for political favours.